KMID : 0360220100510050746
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Journal of the Korean Ophthalmological Society 2010 Volume.51 No. 5 p.746 ~ p.750
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Effect of High Glucose on the Production of Reactive Oxygen Species in R28 Cells
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Lee Jong-Min
Kim Jae-Woo
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Abstract
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Purpose: To investigate the effect of high glucose (HG) on the production of reactive oxygen species (ROS) in retinal precursor R28 cells.
Methods: R28 cells were incubated with low glucose (5 mM) or HG (25 mM) for two days. Additionally, the cells were co-exposed to 50 ¥ìM N-acetyl cysteine or 100 ¥ìM L-arginine. Production of nitric oxide (NO), ROS, and superoxide were assessed by Griess assay, DCFH-DA assay, and modified cytochrome c assay, respectively.
Results: HG increased the production of NO, ROS, and superoxide, which were abolished by antioxidants NAC and L-arginine (a substrate for NO production).
Conclusions: HG increased ROS production in R28 cells. Thus, HG may cause cellular dysfunction and damage by inducing oxidative stress in retinal ganglion cells.
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KEYWORD
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High glucose, Oxidative stress, Reactive oxygen species, Retinal ganglion cells
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